Application
Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP13795a Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Beclin 1 Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Beclin 1 antibody detected the Beclin 1 protein (arrow).| Product Name | Beclin 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GT197 |
|---|---|
| Alternative Names | BECN1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 |
| Molecular Weight(MW) | 51896 Da |
| Function | Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) |
| Entrez Gene | 8678 |
|---|---|
| Nucleotide Accession | NP_003757.1 |
Application
Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP13795a Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Beclin 1 Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Beclin 1 antibody detected the Beclin 1 protein (arrow).| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
