Application
Western blot analysis of Beclin1-BH3 Domain Antibody in mouse stomach tissue lysates (35ug/lane). BECN1 (arrow) was detected using the purified Pab.Application
Beclin1-BH3 Domain Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Beclin1-BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Beclin1-BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Beclin1-BH3 Domain Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GT197 |
|---|---|
| Alternative Names | BECN1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 |
| Molecular Weight(MW) | 51896 Da |
| Function | Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) |
| Entrez Gene | 8678 |
|---|
Application
Western blot analysis of Beclin1-BH3 Domain Antibody in mouse stomach tissue lysates (35ug/lane). BECN1 (arrow) was detected using the purified Pab.
Application
Beclin1-BH3 Domain Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Beclin1-BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Beclin1-BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
