Application
Western blot analysis of anti-hBECN1-Q196 Pab in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab (1:30 dilution).Application
The anti-BECN1 Pab is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Beclin 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GT197 |
|---|---|
| Alternative Names | BECN1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 |
| Molecular Weight(MW) | 51896 Da |
| Function | Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) |
| Entrez Gene | 8678 |
|---|
Application
Western blot analysis of anti-hBECN1-Q196 Pab in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab (1:30 dilution).
Application
The anti-BECN1 Pab is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
