Application
A. Western blot analysis on A431 cell lysate using anti-Beclin-1 RabMAb (Cat. #AJ1087a), dilution 1:5000.Application
B. Immunohistochemical staining of paraffin-embedded human breast tissue using anti-Beclin-1 RabMAb (Cat. #AJ1087a).| Product Name | Beclin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein;Protein GT197 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1733Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GT197 |
|---|---|
| Alternative Names | BECN1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 |
| Molecular Weight(MW) | 51896 Da |
| Function | Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) |
| Entrez Gene | 8678 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Beclin-1 RabMAb (Cat. #AJ1087a), dilution 1:5000.
Application
B. Immunohistochemical staining of paraffin-embedded human breast tissue using anti-Beclin-1 RabMAb (Cat. #AJ1087a).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
