Application
ACTB Antibody (AM1829b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor庐 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.| Product Name | Beta-Actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 137CT26.1.1 |
| Host Species | Mouse |
| Tested Applications | IHCIFWB |
| IHC:1:10~50 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACTB Actin cytoplasmic 1 Beta-actin Actin cytoplasmic 1 N-terminally processed |
|---|---|
| Molecular Weight(MW) | 41737 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein. |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 60 |
|---|
Application
ACTB Antibody (AM1829b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor庐 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.| Application Notes | IHC:1:10~50 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
