Application
Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (36ug/lane). Beta-actin (arrow) was detected using the purified Mab.Application
Beta-actin Monoclonal Antibody (Cat. #AM1021B) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Beta-actin Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Beta-actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 8H10D10 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACTB Actin cytoplasmic 1 Beta-actin Actin cytoplasmic 1 N-terminally processed |
|---|---|
| Molecular Weight(MW) | 41737 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | ACTB recombinant protein is used to produce this monoclonal antibody. |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 60 |
|---|
Application
Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (36ug/lane). Beta-actin (arrow) was detected using the purified Mab.
Application
Beta-actin Monoclonal Antibody (Cat. #AM1021B) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Beta-actin Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
