Application
A. Western blot analysis on A431 cell lysate using anti-Bim RabMAb (Cat. #AJ1100a).Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Bim RabMAb (Cat. #AJ1100a).| Product Name | Bim Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y36 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BIM |
|---|---|
| Molecular Weight(MW) | 22171 Da |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis |
| Tissue Specificity | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Note=Associated with intracytoplasmic membranes (By similarity) Isoform BimL: Mitochondrion. Isoform Bim-alpha1: Mitochondrion. |
| Entrez Gene | 10018 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Bim RabMAb (Cat. #AJ1100a).
Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Bim RabMAb (Cat. #AJ1100a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
