Application
The anti-Bim BH3 domain Pab is used in Western blot to detect Bim BH3 in HL-60 cell lysate.| Product Name | Bim BH3 Domain Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2L11; BIM; Bcl-2-like protein 11; Bcl2-interacting mediator of cell death |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BIM |
|---|---|
| Alternative Names | BCL2L11 BIM Bcl-2-like protein 11 Bcl2-interacting mediator of cell death |
| Molecular Weight(MW) | 22171 Da |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis |
| Tissue Specificity | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Note=Associated with intracytoplasmic membranes (By similarity) Isoform BimL: Mitochondrion. Isoform Bim-alpha1: Mitochondrion. |
| Entrez Gene | 10018 |
|---|
Application
The anti-Bim BH3 domain Pab is used in Western blot to detect Bim BH3 in HL-60 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
