Application
BIRC3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BIRC3 antibody detected the BIRC3 protein (arrow).Application
Western blot analysis of BIRC3 (arrow) using rabbit polyclonal BIRC3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.Application
Confocal immunofluorescent analysis of BIRC3 Antibody (N-term)(Cat#AP14020a) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
BIRC3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | BIRC3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BIRC3; API2; IAP1; MIHC; RNF49; Baculoviral IAP repeat-containing protein 3; Apoptosis inhibitor 2; C-IAP2; IAP homolog C; Inhibitor of apoptosis protein 1; RING finger protein 49; TNFR2-TRAF-signaling complex protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | API2 IAP1 MIHC RNF49 |
|---|---|
| Alternative Names | BIRC3 API2 IAP1 MIHC RNF49 Baculoviral IAP repeat-containing protein 3 Apoptosis inhibitor 2 C-IAP2 IAP homolog C Inhibitor of apoptosis protein 1 RING finger protein 49 TNFR2-TRAF-signaling complex protein 1 |
| Molecular Weight(MW) | 68372 Da |
| Function | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8 |
| Tissue Specificity | Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 330 |
|---|---|
| Nucleotide Accession | NP_001156.1 NP_892007.1 |
Application
BIRC3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BIRC3 antibody detected the BIRC3 protein (arrow).
Application
Western blot analysis of BIRC3 (arrow) using rabbit polyclonal BIRC3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.
Application
Confocal immunofluorescent analysis of BIRC3 Antibody (N-term)(Cat#AP14020a) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
BIRC3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
