Application
A. Western blot analysis on Daudi cell lysate using anti-HIAP-1/BIR3 RabMAb (Cat. #AJ1344a), dilution 1:1,000.Application
B. Immunohistochemical analysis of human normal spleen using anti-HIAP-1/BIR3 RabMAb (Cat. #AJ1344a).| Product Name | HIAP-1 (BIR3) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BIRC3, API2, IAP1, MIHC, RNF49, Baculoviral IAP repeat-containing protein 3, RING finger protein 49 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E40 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | API2 IAP1 MIHC RNF49 |
|---|---|
| Alternative Names | BIRC3 API2 IAP1 MIHC RNF49 Baculoviral IAP repeat-containing protein 3 RING finger protein 49 |
| Molecular Weight(MW) | 68372 Da |
| Function | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8 |
| Tissue Specificity | Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 330 |
|---|
Application
A. Western blot analysis on Daudi cell lysate using anti-HIAP-1/BIR3 RabMAb (Cat. #AJ1344a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of human normal spleen using anti-HIAP-1/BIR3 RabMAb (Cat. #AJ1344a).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
