Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human breast tissue(A), lymph tissue(B) and skin carcinoma(C), showing membrane localization using anti-BLK antibody with DAB staining.| Product Name | BLK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC10442 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 9D10D1 |
| Host Species | Mouse |
| Tested Applications | IHC |
| IHC:1:200 - 1000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MGC10442 |
|---|---|
| Molecular Weight(MW) | 57706 Da |
| Function | Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr- 207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose |
| Tissue Specificity | Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles |
| Cellular Localization | Cell membrane; Lipid-anchor (By similarity). Note=Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity) |
| Entrez Gene | 640 |
|---|---|
| Nucleotide Accession | NP_001706.2 |
Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human breast tissue(A), lymph tissue(B) and skin carcinoma(C), showing membrane localization using anti-BLK antibody with DAB staining.| Application Notes | IHC:1:200 - 1000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of BLK expressed in E. Coli. |
