Application
Western blot analysis of BLM Antibody (C-term) in HL-60 cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.Application
BLM Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BLM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase, RecQ-like type 2; RecQ protein-like 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RECQ2 RECQL3 |
|---|---|
| Alternative Names | BLM RECQ2 RECQL3 Bloom syndrome protein DNA helicase RecQ-like type 2 RecQ protein-like 3 |
| Molecular Weight(MW) | 159000 Da |
| Function | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA |
| Tissue Specificity | This BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061~1090 amino acids from the C-terminal region of human BLM. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 641 |
|---|
Application
Western blot analysis of BLM Antibody (C-term) in HL-60 cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.
Application
BLM Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
