Application
Western blot analysis of BLM Antibody (C-term) in Jurkat cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.Application
BLM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
BLM Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BLM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase, RecQ-like type 2; RecQ protein-like 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RECQ2 RECQL3 |
|---|---|
| Alternative Names | BLM RECQ2 RECQL3 Bloom syndrome protein DNA helicase RecQ-like type 2 RecQ protein-like 3 |
| Molecular Weight(MW) | 159000 Da |
| Function | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA |
| Tissue Specificity | This BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1316~1346 amino acids from the C-terminal region of human BLM. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 641 |
|---|
Application
Western blot analysis of BLM Antibody (C-term) in Jurkat cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.
Application
BLM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
BLM Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
