Application
BMP4 (Cat. #AM1872a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BMP4 antibody detected the BMP4 protein (arrow).Application
BMP4 Monoclonal( Ascites) (Cat. #AM1872a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BMP4 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of BMP4 Antibody (Ascites)(Cat#AM1872a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | BMP4 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMP4; BMP2B; DVR4; Bone morphogenetic protein 4; Bone morphogenetic protein 2B |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 192CT7.2.6 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500~16000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BMP2B DVR4 |
|---|---|
| Alternative Names | BMP4 BMP2B DVR4 Bone morphogenetic protein 4 Bone morphogenetic protein 2B |
| Molecular Weight(MW) | 46555 Da |
| Function | Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity) |
| Tissue Specificity | Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 652 |
|---|---|
| Nucleotide Accession | NP_570912.2 NP_001193.2 |
Application
BMP4 (Cat. #AM1872a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BMP4 antibody detected the BMP4 protein (arrow).
Application
BMP4 Monoclonal( Ascites) (Cat. #AM1872a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BMP4 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of BMP4 Antibody (Ascites)(Cat#AM1872a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~16000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
