Application
BRAP Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRAP antibody detected the BRAP protein (arrow).| Product Name | BRAP (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAP; RNF52; BRCA1-associated protein; BRAP2; Impedes mitogenic signal propagation; RING finger protein 52; Renal carcinoma antigen NY-REN-63 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF52 |
|---|---|
| Alternative Names | BRAP RNF52 BRCA1-associated protein BRAP2 Impedes mitogenic signal propagation RING finger protein 52 Renal carcinoma antigen NY-REN-63 |
| Molecular Weight(MW) | 67305 Da |
| Function | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport |
| Tissue Specificity | Expressed in breast epithelial cell lines. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 8315 |
|---|---|
| Nucleotide Accession | NP_006759.3 |
Application
BRAP Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRAP antibody detected the BRAP protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
