Application
BRAP Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the BRAP antibody detected BRAP protein (arrow).Application
BRAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BRAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
BRAP Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BRAP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAP; RNF52; BRCA1-associated protein; BRAP2; Impedes mitogenic signal propagation; RING finger protein 52; Renal carcinoma antigen NY-REN-63 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF52 |
|---|---|
| Alternative Names | BRAP RNF52 BRCA1-associated protein BRAP2 Impedes mitogenic signal propagation RING finger protein 52 Renal carcinoma antigen NY-REN-63 |
| Molecular Weight(MW) | 67305 Da |
| Function | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport |
| Tissue Specificity | Expressed in breast epithelial cell lines. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 8315 |
|---|---|
| Nucleotide Accession | NP_006759.3 |
Application
BRAP Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the BRAP antibody detected BRAP protein (arrow).
Application
BRAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BRAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
BRAP Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
