Application
Figure 1: Western blot analysis using anti-BTK monoclonal antiobdy against K562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using anti-BTK antibody with DAB staining.Application
Figure 3: Immunofluorescence analysis of Jurkat cells using anti-BTK monoclonal antibody.| Product Name | BTK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F12H4 |
| Host Species | Mouse |
| Tested Applications | WB,IHC,IF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AGMX1, ATK, BPK |
|---|---|
| Alternative Names | BTK |
| Molecular Weight(MW) | 76281 Da |
| Function | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis |
| Tissue Specificity | Predominantly expressed in B-lymphocytes. |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1 |
| Entrez Gene | 695 |
|---|---|
| Nucleotide Accession | NP_000052.1 |
Application
Figure 1: Western blot analysis using anti-BTK monoclonal antiobdy against K562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using anti-BTK antibody with DAB staining.
Application
Figure 3: Immunofluorescence analysis of Jurkat cells using anti-BTK monoclonal antibody.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of BTK expressed in E. Coli. |
