Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissueusing C-Cbl Antibody (Y770) (#AE1007a).| Product Name | C-Cbl Antibody (Y770) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RING finger protein 55; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; Signal transduction protein CBL; E3 ubiquitin-protein ligase CBL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CBL2 RNF55 |
|---|---|
| Alternative Names | RING finger protein 55 Casitas B-lineage lymphoma proto-oncogene Proto-oncogene c-Cbl Signal transduction protein CBL E3 ubiquitin-protein ligase CBL |
| Molecular Weight(MW) | 99633 Da |
| Function | Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling Recognizes membrane-bound HCK and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The Tyr-731 phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane |
| Entrez Gene | 867 |
|---|
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissueusing C-Cbl Antibody (Y770) (#AE1007a).| Application Notes | IHC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
