Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Cbl (pY674) RabMAb (Cat. #AJ1142c), 1:1000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | Cbl Antibody Phospho (pY674) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBL, CBL2, RNF55, E3 ubiquitin-protein ligase CBL, Signal transduction protein CBL;Proto-oncogene c-CBL;Casitas B-lineage lymphoma proto-oncogene;RING finger protein 55 |
| Modification | p-Y674 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2227 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CBL2 RNF55 |
|---|---|
| Alternative Names | CBL CBL2 RNF55 E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-CBL Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 |
| Molecular Weight(MW) | 99633 Da |
| Function | Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling Recognizes membrane-bound HCK and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The Tyr-731 phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding tyrosine 674 of human Cbl were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 674. |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane |
| Entrez Gene | 867 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Cbl (pY674) RabMAb (Cat. #AJ1142c), 1:1000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
