Application
Western blot analysis of CA5A Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CA5A (arrow) was detected using the purified Pab.Application
CA5A Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CA5A Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CA5A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CA5A; CA5; Carbonic anhydrase 5A, mitochondrial; Carbonate dehydratase VA; Carbonic anhydrase VA |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CA5 |
|---|---|
| Alternative Names | CA5A CA5 Carbonic anhydrase 5A mitochondrial Carbonate dehydratase VA Carbonic anhydrase VA |
| Molecular Weight(MW) | 34750 Da |
| Function | Reversible hydration of carbon dioxide. Low activity. |
| Tissue Specificity | This CA5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~224 amino acids from the Central region of human CA5A. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 763 |
|---|
Application
Western blot analysis of CA5A Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CA5A (arrow) was detected using the purified Pab.
Application
CA5A Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CA5A Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM228466