Application
A. Western blot analysis on NIH3T3 cell lysate using anti-Caldesmon RabMAb (Cat. #AJ1117a); dilution 1:20,000.Application
B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Caldesmon RabMAb (Cat. #AJ1117a).Application
C. Immunofluorescent staining of HeLa cells using anti-Caldesmon RabMAb (Cat. #AJ1117a).| Product Name | Caldesmon Antibody (CaM) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CALD1, CAD, CDM, Caldesmon |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E89 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAD CDM |
|---|---|
| Alternative Names | CALD1 CAD CDM Caldesmon |
| Molecular Weight(MW) | 93231 Da |
| Function | Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping Involved in Schwann cell migration during peripheral nerve regeneration (By similarity) |
| Tissue Specificity | High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart |
| Cellular Localization | Cytoplasm, cytoskeleton (By similarity). Cytoplasm, myofibril (By similarity). Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity) |
| Entrez Gene | 800 |
|---|
Application
A. Western blot analysis on NIH3T3 cell lysate using anti-Caldesmon RabMAb (Cat. #AJ1117a); dilution 1:20,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Caldesmon RabMAb (Cat. #AJ1117a).
Application
C. Immunofluorescent staining of HeLa cells using anti-Caldesmon RabMAb (Cat. #AJ1117a).| Application Notes | WB:1:500~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
