Application
Figure 1: Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.Application
Figure 2: Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).| Product Name | Calnexin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNX; P90; CANX |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 3H4A7 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CNX P90 CANX |
|---|---|
| Molecular Weight(MW) | 67568 Da |
| Function | Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse |
| Tissue Specificity | Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. The palmitoylated form preferentially localizes to the perinuclear rough ER |
| Entrez Gene | 821 |
|---|---|
| Nucleotide Accession | NP_001737.1 |
Application
Figure 1: Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.
Application
Figure 2: Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH. |
