Application
Western blot analysis of CAMK2gamma-P397 in Jurkat, T47D cell line lysates (35ug/lane). CAMK2gamma (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Confocal immunofluorescent analysis of CAMK2G (CAMK2 gamma) Antibody (C-term)(Cat#AP7210a) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CAMK2G (CAMK2 gamma) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAMK2G; CAMK; CAMK-II; CAMKG; Calcium/calmodulin-dependent protein kinase type II subunit gamma |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAMK CAMK-II CAMKG |
|---|---|
| Alternative Names | CAMK2G CAMK CAMK-II CAMKG Calcium/calmodulin-dependent protein kinase type II subunit gamma |
| Molecular Weight(MW) | 62609 Da |
| Function | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow- twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning |
| Tissue Specificity | Expressed in skeletal muscle. |
| Cellular Localization | Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (Probable) |
| Entrez Gene | 818 |
|---|
Application
Western blot analysis of CAMK2gamma-P397 in Jurkat, T47D cell line lysates (35ug/lane). CAMK2gamma (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Confocal immunofluorescent analysis of CAMK2G (CAMK2 gamma) Antibody (C-term)(Cat#AP7210a) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
