Application
Western blot analysis of CASP1 antibody (Center) in HL60 cell line lysates (35ug/lane). CASP1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung tissue with CASP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using CASP1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CASP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP1; IL1BC; IL1BCE; Caspase-1; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; Caspase-1 subunit p20; Caspase-1 subunit p10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL1BC IL1BCE |
|---|---|
| Alternative Names | CASP1 IL1BC IL1BCE Caspase-1 Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 Caspase-1 subunit p20 Caspase-1 subunit p10 |
| Molecular Weight(MW) | 45159 Da |
| Function | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis |
| Tissue Specificity | Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 834 |
|---|
Application
Western blot analysis of CASP1 antibody (Center) in HL60 cell line lysates (35ug/lane). CASP1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung tissue with CASP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using CASP1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
