Application
Western blot analysis of CASP7 antibody (Center) (Cat.# AP1328b) in 293 cell line lysates (35ug/lane). CASP7 (arrow) was detected using the purified Pab.Application
Western blot analysis of CASP7 (arrow) using rabbit polyclonal CASP7 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP7 gene.Application
Confocal immunofluorescent analysis of CASP7 Antibody (Center)(Cat#AP1328b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CASP7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP7; MCH3; Caspase-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; Caspase-7 subunit p20; Caspase-7 subunit p11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH3 |
|---|---|
| Alternative Names | CASP7 MCH3 Caspase-7 Apoptotic protease Mch-3 CMH-1 ICE-like apoptotic protease 3 Caspase-7 subunit p20 Caspase-7 subunit p11 |
| Molecular Weight(MW) | 34277 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death |
| Tissue Specificity | Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 840 |
|---|
Application
Western blot analysis of CASP7 antibody (Center) (Cat.# AP1328b) in 293 cell line lysates (35ug/lane). CASP7 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CASP7 (arrow) using rabbit polyclonal CASP7 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP7 gene.
Application
Confocal immunofluorescent analysis of CASP7 Antibody (Center)(Cat#AP1328b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
