Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-CASP9 Antibody (Center) in CEM cell line lysates (35ug/lane).CASP9(arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CASP9 Antibody (Center)(Cat#AP7974c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | CASP9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP9; MCH6; Caspase-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; Caspase-9 subunit p35; Caspase-9 subunit p10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH6 |
|---|---|
| Alternative Names | CASP9 MCH6 Caspase-9 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 ICE-like apoptotic protease 6 Caspase-9 subunit p35 Caspase-9 subunit p10 |
| Molecular Weight(MW) | 46281 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP) |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes |
| Entrez Gene | 842 |
|---|
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-CASP9 Antibody (Center) in CEM cell line lysates (35ug/lane).CASP9(arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CASP9 Antibody (Center)(Cat#AP7974c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
