Application
A. Western blot analysis on Jurkat cell lysate using anti-Pro-Caspase-9 RabMAb (Cat. #AJ1136b), 1:1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (Cat. #AJ1136b).Application
C. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-9 RabMAb (red) (Cat. #AJ1136b) or a rabbit IgG (negative) (green).| Product Name | Caspase-9 Antibody (Pro) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP9, MCH6, Caspase-9 subunit p10, Apoptotic protease Mch-6;Apoptotic protease-activating factor 3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E84 |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH6 |
|---|---|
| Alternative Names | CASP9 MCH6 Caspase-9 subunit p10 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 |
| Molecular Weight(MW) | 46281 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP) |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes |
| Entrez Gene | 842 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Pro-Caspase-9 RabMAb (Cat. #AJ1136b), 1:1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (Cat. #AJ1136b).
Application
C. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-9 RabMAb (red) (Cat. #AJ1136b) or a rabbit IgG (negative) (green).| Application Notes | WB:1:500~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
