Application
CD11b antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD11b antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CD11b Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
CD11b Antibody (N-term) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).| Product Name | CD11b Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | HumanMousePorcineRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD11B, CR3A |
|---|---|
| Alternative Names | ITGAM CD11B CR3A Integrin alpha-M CD11 antigen-like family member B CR-3 alpha chain Cell surface glycoprotein MAC-1 subunit alpha Leukocyte adhesion receptor MO1 Neutrophil adherence receptor CD_antigen=CD11b Flags: Precursor |
| Molecular Weight(MW) | 127179 Da |
| Function | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain |
| Tissue Specificity | Predominantly expressed in monocytes and granulocytes |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
CD11b antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD11b antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CD11b Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
CD11b Antibody (N-term) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
