Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human lymphnode, showing membrane localization using anti-CD14 antibody with DAB staining.Application
Figure 2: Flow cytometric analysis of human PBMC using anti-CD14 monoclonal antibody.| Product Name | CD14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A3 |
| Host Species | Mouse |
| Tested Applications | IHCFC |
| IHC:1:200 - 400 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD14 |
|---|---|
| Molecular Weight(MW) | 40076 Da |
| Function | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules |
| Tissue Specificity | Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Entrez Gene | 929 |
|---|---|
| Nucleotide Accession | NP_001035110.1 |
Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human lymphnode, showing membrane localization using anti-CD14 antibody with DAB staining.
Application
Figure 2: Flow cytometric analysis of human PBMC using anti-CD14 monoclonal antibody.| Application Notes | IHC:1:200 - 400 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human CD14 expressed in E. Coli. |
