Application
Figure 1: Confocal immunofluorescence analysis of HL60 cells using anti-CD18 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 2: Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using anti-CD18 monoclonal antioby (red). Blue: DRAQ5 fluorescent DNA dye.| Product Name | CD18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 1.0E+13 |
| Host Species | Mouse |
| Tested Applications | IF |
| IF:1:200 - 1000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD18, MFI7 |
|---|---|
| Alternative Names | LAD MF17 LCAMB LFA-1 ITGB2 |
| Molecular Weight(MW) | 84782 Da |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation |
| Tissue Specificity | Purified recombinant fragment of CD18 expressed in E. Coli. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3689 |
|---|---|
| Nucleotide Accession | NP_001120963.1 |
Application
Figure 1: Confocal immunofluorescence analysis of HL60 cells using anti-CD18 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 2: Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using anti-CD18 monoclonal antioby (red). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | IF:1:200 - 1000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of CD18 expressed in E. Coli. |
