Application
The anti-Siglec3 N-term Pab is used in Western blot to detect Siglec3 in jurkat cell lysate.Application
Formalin-fixed and paraffin-embedded human lymph with CD33 (SIGLEC3) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CD33 (SIGLEC3) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; gp67; CD_antigen=CD33; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIGLEC3 |
|---|---|
| Alternative Names | CD33 SIGLEC3 Myeloid cell surface antigen CD33 Sialic acid-binding Ig-like lectin 3 gp67 CD_antigen=CD33 Flags: Precursor |
| Molecular Weight(MW) | 39825 Da |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro) |
| Tissue Specificity | Monocytic/myeloid lineage cells. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 945 |
|---|
Application
The anti-Siglec3 N-term Pab is used in Western blot to detect Siglec3 in jurkat cell lysate.
Application
Formalin-fixed and paraffin-embedded human lymph with CD33 (SIGLEC3) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
