Application
Figure 1: Western blot analysis using anti-CD33 monoclonal antibody against truncated Trx-CD33 recombinant protein(1),truncated CD33(aa48-258)-His recombinant protein(2) and truncated CD33(aa18-259)-hIgGFc transfected CHO-K1 cell lysate(3).| Product Name | CD33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p67; SIGLEC3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2B7C12 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIGLEC3 |
|---|---|
| Alternative Names | p67 SIGLEC3 |
| Molecular Weight(MW) | 39825 Da |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro) |
| Tissue Specificity | Monocytic/myeloid lineage cells. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 945 |
|---|---|
| Nucleotide Accession | NP_001763.3 |
Application
Figure 1: Western blot analysis using anti-CD33 monoclonal antibody against truncated Trx-CD33 recombinant protein(1),truncated CD33(aa48-258)-His recombinant protein(2) and truncated CD33(aa18-259)-hIgGFc transfected CHO-K1 cell lysate(3).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of CD33(48-258) expressed in E. Coli. |
