Application
CD33 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow).Application
Western blot analysis of CD33 (arrow) using rabbit polyclonal CD33 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD33 gene.| Product Name | CD33 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; gp67; CD_antigen=CD33; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIGLEC3 |
|---|---|
| Alternative Names | CD33 SIGLEC3 Myeloid cell surface antigen CD33 Sialic acid-binding Ig-like lectin 3 gp67 CD_antigen=CD33 Flags: Precursor |
| Molecular Weight(MW) | 39825 Da |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro) |
| Tissue Specificity | Monocytic/myeloid lineage cells. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 945 |
|---|---|
| Nucleotide Accession | NP_001763.3 |
Application
CD33 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow).
Application
Western blot analysis of CD33 (arrow) using rabbit polyclonal CD33 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD33 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
