Application
Western blot analysis of anti-CD34 Antibody (Center) (Cat. #AM1825a) in mouse NIH-3T3 cell line lysates (35ug/lane). CD34 (arrow) was detected using the purified Mab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD34 Monoclonal Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CD34 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD34; Hematopoietic progenitor cell antigen CD34; CD_antigen=CD34; Flags: Precursor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 132CT29.1.5 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD34 Hematopoietic progenitor cell antigen CD34 CD_antigen=CD34 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 40716 Da |
| Function | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins |
| Tissue Specificity | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Western blot analysis of anti-CD34 Antibody (Center) (Cat. #AM1825a) in mouse NIH-3T3 cell line lysates (35ug/lane). CD34 (arrow) was detected using the purified Mab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD34 Monoclonal Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:500-1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
