Application
Figure 1: Flow cytometric analysis of peripheral blood T cells using anti-CD4 monoclonal antibody| Product Name | CD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD4mut |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | B486A1 |
| Host Species | Mouse |
| Tested Applications | FC |
| FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD4mut |
|---|---|
| Molecular Weight(MW) | 51111 Da |
| Function | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum |
| Entrez Gene | 920 |
|---|---|
| Nucleotide Accession | NP_000607.1 |
Application
Figure 1: Flow cytometric analysis of peripheral blood T cells using anti-CD4 monoclonal antibody| Application Notes | FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human CD4 expressed in E. Coli. |
