Application
CD4 Antibody (Ascites)(Cat. #AM1957a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CD4 antibody detected the CD4 protein (arrow).| Product Name | CD4 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen=CD4; Flags: Precursor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | OKT4 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD4 T-cell surface glycoprotein CD4 T-cell surface antigen T4/Leu-3 CD_antigen=CD4 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 51111 Da |
| Function | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts |
| Tissue Specificity | This CD4 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from human CD4. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum |
| Entrez Gene | 920 |
|---|---|
| Nucleotide Accession | NP_000607.1 |
Application
CD4 Antibody (Ascites)(Cat. #AM1957a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CD4 antibody detected the CD4 protein (arrow).| Application Notes | WB:1:100~800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
