Application
Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody (M01), clone 5D5.Lane 1: CD97 transfected lysate(81.7 KDa).Lane 2: Non-transfected lysate.Application
Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi ( Cat # H00000976-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody (M01), clone 5D5 (Cat # AT1451a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | CD97 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TM7LN1CD97 antigen |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5D5 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TM7LN1CD97 antigen |
|---|---|
| Molecular Weight(MW) | 91869 Da |
| Tissue Specificity | CD97 (NP_510966, 421 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody (M01), clone 5D5.Lane 1: CD97 transfected lysate(81.7 KDa).Lane 2: Non-transfected lysate.
Application
Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi ( Cat # H00000976-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody (M01), clone 5D5 (Cat # AT1451a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
