Application
Western blot analysis of CDC25C (arrow) using CDC25C Antibody (Cat. #AM1908b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC25C gene.| Product Name | CDC25C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 233CT9.6.6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC25C M-phase inducer phosphatase 3 Dual specificity phosphatase Cdc25C |
|---|---|
| Molecular Weight(MW) | 53365 Da |
| Function | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity |
| Tissue Specificity | This CDC25C monoclonal antibody is generated from mouse immunized with CDC25C recombinant protein. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 995 |
|---|---|
| Nucleotide Accession | NP_001781.2 NP_073720.1 |
Application
Western blot analysis of CDC25C (arrow) using CDC25C Antibody (Cat. #AM1908b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC25C gene.| Application Notes | WB:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
