Application
Western blot analysis of CENPN Antibody (C-term) in MCF-7 cell line lysates (35ug/lane).CENPN (arrow) was detected using the purified Pab.Application
CENPN Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CENPN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPN; C16orf60; ICEN32; Centromere protein N; Interphase centromere complex protein 32 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C16orf60 ICEN32 |
|---|---|
| Alternative Names | CENPN C16orf60 ICEN32 Centromere protein N Interphase centromere complex protein 32 |
| Molecular Weight(MW) | 39555 Da |
| Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate |
| Tissue Specificity | This CENPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-339 amino acids from the C-terminal region of human CENPN. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis |
| Entrez Gene | 55839 |
|---|
Application
Western blot analysis of CENPN Antibody (C-term) in MCF-7 cell line lysates (35ug/lane).CENPN (arrow) was detected using the purified Pab.
Application
CENPN Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
