Application
Western blot analysis of CENPN Antibody (Center) in MDA-MB468 cell line lysates (35ug/lane). CENPN (arrow) was detected using the purified Pab.Application
CENPN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CENPN Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPN; C16orf60; ICEN32; Centromere protein N; Interphase centromere complex protein 32 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C16orf60 ICEN32 |
|---|---|
| Alternative Names | CENPN C16orf60 ICEN32 Centromere protein N Interphase centromere complex protein 32 |
| Molecular Weight(MW) | 39555 Da |
| Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate |
| Tissue Specificity | This CENPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 125-155 from the Central region of human CENPN. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis |
| Entrez Gene | 55839 |
|---|
Application
Western blot analysis of CENPN Antibody (Center) in MDA-MB468 cell line lysates (35ug/lane). CENPN (arrow) was detected using the purified Pab.
Application
CENPN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
