Application
CHAF1A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CHAF1A antibody detected the CHAF1A protein (arrow).Application
CHAF1A Antibody (C-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the CHAF1A antibody detected CHAF1A protein (arrow).Application
CHAF1A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CHAF1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; Chromatin assembly factor I p150 subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAF CAF1P150 |
|---|---|
| Alternative Names | CHAF1A CAF CAF1P150 Chromatin assembly factor 1 subunit A Chromatin assembly factor I p150 subunit |
| Molecular Weight(MW) | 106926 Da |
| Function | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene |
| Tissue Specificity | This CHAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-657 amino acids from the C-terminal region of human CHAF1A. |
| Cellular Localization | Nucleus. Note=DNA replication foci. |
Application
CHAF1A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CHAF1A antibody detected the CHAF1A protein (arrow).
Application
CHAF1A Antibody (C-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the CHAF1A antibody detected CHAF1A protein (arrow).
Application
CHAF1A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
