Application
Figure 1: Western blot analysis using anti-CK17 monoclonal antibody against Hela (1), MCF-7 (2) and A431 (3) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), endometrial carcinoma (B), sublingual gland (C) and esophagus (D) tissues using anti-CK17 antibody with DAB staining.| Product Name | CK17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PC; K17; PC2; PCHC1; KRT17 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 3B12 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PC K17 PC2 PCHC1 KRT17 |
|---|---|
| Molecular Weight(MW) | 48106 Da |
| Function | May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair (By similarity) |
| Tissue Specificity | Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level) |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 3872 |
|---|---|
| Nucleotide Accession | NP_000413.1 |
Application
Figure 1: Western blot analysis using anti-CK17 monoclonal antibody against Hela (1), MCF-7 (2) and A431 (3) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), endometrial carcinoma (B), sublingual gland (C) and esophagus (D) tissues using anti-CK17 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of CK17 expressed in E. Coli. |
