Application
Figure 1: Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.Application
Figure 2:Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | CLOCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KAT13D; bHLHe8; KIAA0334; CLOCK |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8F7 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE8 KIAA0334 |
|---|---|
| Alternative Names | KAT13D bHLHe8 KIAA0334 CLOCK |
| Molecular Weight(MW) | 95304 Da |
| Function | ARNTL/2-CLOCK heterodimers activate E-box element (5'- CACGTG-3') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL (By similarity). Plays a role in DNA damage response (DDR) signaling during the S phase |
| Tissue Specificity | Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN) |
| Cellular Localization | Nucleus. Chromosome. Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus (By similarity). Localizes to sites of DNA damage in a H2AX- independent manner |
| Entrez Gene | 9575 |
|---|---|
| Nucleotide Accession | NP_004889.1 |
Application
Figure 1: Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.
Application
Figure 2:Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human CLOCK expressed in E. Coli. |
