Application
COP1 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
COP1 Antibody (N-term) western blot analysis in CEM,A549 cell line lysates (35ug/lane).This demonstrates the COP1 antibody detected the COP1 protein (arrow).| Product Name | COP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARD16; COP; COP1; Caspase recruitment domain-containing protein 16; CARD only domain-containing protein 1; Caspase-1 inhibitor COP; Pseudo interleukin-1 beta converting enzyme |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| FC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COP COP1 |
|---|---|
| Alternative Names | CARD16 COP COP1 Caspase recruitment domain-containing protein 16 CARD only domain-containing protein 1 Caspase-1 inhibitor COP Pseudo interleukin-1 beta converting enzyme |
| Molecular Weight(MW) | 22625 Da |
| Function | Caspase inhibitor. Acts as a regulator of procaspase- 1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro- inflammatory cytokine response. Also able to inhibit CASP1- mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2 |
| Tissue Specificity | Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus |
| Entrez Gene | 114769 |
|---|---|
| Nucleotide Accession | NP_443121.1 NP_001017534.1 |
Application
COP1 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
COP1 Antibody (N-term) western blot analysis in CEM,A549 cell line lysates (35ug/lane).This demonstrates the COP1 antibody detected the COP1 protein (arrow).| Application Notes | FC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
