Application
CRHR2 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CRHR2 Antibody (Center) in Hela cell line lysates (35ug/lane). CRHR2 (arrow) was detected using the purified Pab.Application
CRHR2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRHR2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of CRHR2 Antibody (Center)(Cat#AP6787c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CRHR2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRHR2; CRF2R; CRH2R; Corticotropin-releasing factor receptor 2; Corticotropin-releasing hormone receptor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCWBIHCIF |
| FC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRF2R CRH2R |
|---|---|
| Alternative Names | CRHR2 CRF2R CRH2R Corticotropin-releasing factor receptor 2 Corticotropin-releasing hormone receptor 2 |
| Molecular Weight(MW) | 47688 Da |
| Function | This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase |
| Tissue Specificity | This CRHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250~280 amino acids from the Center region of human CRHR2. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| Entrez Gene | 1395 |
|---|
Application
CRHR2 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CRHR2 Antibody (Center) in Hela cell line lysates (35ug/lane). CRHR2 (arrow) was detected using the purified Pab.
Application
CRHR2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRHR2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of CRHR2 Antibody (Center)(Cat#AP6787c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | FC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
