Application
A. Western blot analysis on (A) fetal uterus, (B) HeLa, (C) A375 and (D) fetal testis lysates using anti-CUL4A/B RabMAb (Cat. #AJ1207b), dilution 1:5000.Application
B. Immunohistochemical staining of paraffin-embedded brain tissue using anti-CUL4A/B RabMAb (Cat. #AJ1207b).| Product Name | CUL4A/B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KHDRBS1, SAM68, KH domain-containing, RNA-binding, signal transduction-associated protein 1, p68 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3200 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0695 |
|---|---|
| Alternative Names | KHDRBS1 SAM68 KH domain-containing RNA-binding signal transduction-associated protein 1 p68 |
| Molecular Weight(MW) | 103982 Da |
| Function | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 |
| Tissue Specificity | A synthetic peptide corresponding to residues in human CUL4B was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8450 |
|---|
Application
A. Western blot analysis on (A) fetal uterus, (B) HeLa, (C) A375 and (D) fetal testis lysates using anti-CUL4A/B RabMAb (Cat. #AJ1207b), dilution 1:5000.
Application
B. Immunohistochemical staining of paraffin-embedded brain tissue using anti-CUL4A/B RabMAb (Cat. #AJ1207b).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
