Application
CUL4B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CUL4B antibody detected the CUL4B protein (arrow).| Product Name | CUL4B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL4B; KIAA0695; Cullin-4B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0695 |
|---|---|
| Alternative Names | CUL4B KIAA0695 Cullin-4B |
| Molecular Weight(MW) | 103982 Da |
| Function | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 |
| Tissue Specificity | This CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-260 amino acids from the Central region of human CUL4B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8450 |
|---|---|
| Nucleotide Accession | NP_003579.3 |
Application
CUL4B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CUL4B antibody detected the CUL4B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
