Application
Western blot analysis of anti-CUL5 Antibody (C-term) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
CUL5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUL5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CUL5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL5; VACM1; Cullin-5; Vasopressin-activated calcium-mobilizing receptor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VACM1 |
|---|---|
| Alternative Names | CUL5 VACM1 Cullin-5 Vasopressin-activated calcium-mobilizing receptor 1 |
| Molecular Weight(MW) | 90955 Da |
| Function | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor |
| Tissue Specificity | This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751~780 amino acids from the C-terminal region of human CUL5. |
| Entrez Gene | 8065 |
|---|
Application
Western blot analysis of anti-CUL5 Antibody (C-term) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
CUL5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUL5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
