Application
Western blot analysis of anti-CUL5 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CUL5(arrow) was detected using the purified Pab .Application
Formalin-fixed and paraffin-embedded human testis tissue with CUL5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CUL5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL5; VACM1; Cullin-5; Vasopressin-activated calcium-mobilizing receptor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VACM1 |
|---|---|
| Alternative Names | CUL5 VACM1 Cullin-5 Vasopressin-activated calcium-mobilizing receptor 1 |
| Molecular Weight(MW) | 90955 Da |
| Function | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor |
| Tissue Specificity | This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~263 amino acids from the center region of human CUL5. |
| Entrez Gene | 8065 |
|---|
Application
Western blot analysis of anti-CUL5 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CUL5(arrow) was detected using the purified Pab .
Application
Formalin-fixed and paraffin-embedded human testis tissue with CUL5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
