Application
Western blot analysis of anti-CXADR Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CXADR(arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H460 cells using CXADR Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CXADR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CXADR; CAR; Coxsackievirus and adenovirus receptor; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAR |
|---|---|
| Alternative Names | CXADR CAR Coxsackievirus and adenovirus receptor CVB3-binding protein Coxsackievirus B-adenovirus receptor HCVADR |
| Molecular Weight(MW) | 40030 Da |
| Function | Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN |
| Tissue Specificity | Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Basolateral cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface Isoform 3: Secreted. Isoform 5: Secreted. |
| Entrez Gene | 1525 |
|---|
Application
Western blot analysis of anti-CXADR Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CXADR(arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H460 cells using CXADR Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
